PRF와 연조직 이식술

Clin Oral Investig. 2016 Nov;20(8):2045-2053. Epub 2015 Dec 23.

Vascularization after treatment of gingival recession defects with platelet-rich fibrin or connective tissue graft.

Eren G¹, Kantarcı A², Sculean A³, Atilla G⁴.

Author information

• ¹School of Dentistry, Department of Periodontology, Ege University, Bornova-İzmir, Turkey. gulnihal_karasu@hotmail.com.
• ²Department of Applied Oral Sciences, Center for Periodontology, The Forsyth Institute, Cambridge, MA, 02142, USA.
• ³Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
• ⁴School of Dentistry, Department of Periodontology, Ege University, Bornova-İzmir, Turkey.

Abstract

OBJECTIVE:

The aim of this study was to evaluate histologically the following treatment of bilateral localized gingival recessions with coronally advanced flap (CAF) combined with platelet-rich fibrin (PRF) or subepithelial connective tissue graft (SCTG).

MATERIALS AND METHODS:

Tissue samples were harvested from 14 subjects either 1 or 6 months after the surgeries. The 2-mm punch biopsies were obtained from the mid-portion of the grafted sites. Neutral buffered formalin fixed, paraffin-embedded 5-μm thick tissue sections were stained with hematoxylin eosin and Masson's trichrome in order to analyze the collagen framework, epithelium thickness and rete-peg length. Multiple sequential sections were cut from paraffin-embedded blocks of tissue and immunohistochemically prepared for detection of vascular endothelial growth factor, CD31 and CD34, for the assessment of vascularization.

RESULTS:

Rete peg formation was significantly increased in the sites treated with PRF compared to the SCTG group after 6 months (p < 0.05). on the contrary, the number of vessels was increased in the SCTG group compared to the PRF group after 6 months (p < 0.05). No statistically significant differences were observed in the collagen density. Staining intensity of CD31 increased in submucosal area of PRF group than SCTG group after 1 month. Higher staining intensity of CD34 was observed in the submucosal area of PRF group compared with SCTG group after 6 months.

CONCLUSIONS:

The results of the present study suggest that in histological evaluation because of its biological compounds, PRF results earlier vessel formation and tissue maturation compared to connective tissue graft.

CLINICAL RELEVANCE:

PRF regulated the vascular response associated with an earlier wound healing.

KEYWORDS:

Gingival recession; Histology; Platelet-rich fibrin; Subepithelial connective tissue graft; Wound healing