nHAP를 이용한 탈회된 치면의 수복(재광화)

電解放出型走査電子顕微鏡観察下での薬用ハイドロキシアパタイト配合歯磨材
を用いたブラッシング後の脱灰エナメル質表面形態について
西村一行、山口芳功、吉武一貞
日本口腔科学会雑誌、第48 巻、第3 号、199-210、1999

＜Abstract＞
The deposition from of hydroxyapatite on the enamel surface cannot be observed
under high magnification with conventional SEM because of charging of the tooth.
However, using FE-SEM, which made it possible to maintain a low acceleration voltage,
minute structures of the enamel surface have been observed under high magnification.
These observations were made without damaging the specimen or charging the tooth.
This study was carried out to investigate the influence of medical hydroxyapatyte,
which acted on the decalcified enamel surface of teeth microstructurally. The enamel
surface of sound, caries-free human molars was decalcified with 0.1M HCL solution for
five minutes in vitro. The enamel surface was treated by toothpaste containing medical
hydroxyapatite, a control toothpaste, and a popular toothpaste. The surface from and
crystal from was observed using FE-SEM, and the results were compared. The enamel
surface treated with toothpaste containing medical hydroxyapatite was smooth under
low magnification, and the gap of the enamel prism was covered by minute crystal
particles(30-60nm) in a high magnification FE-SEM image. The enamel surface treated
with the control toothpaste and the popular brand of toothpaste was not smooth under
low maginihications, and an enamel prism from was confirmed in a high magnification
FE-SEM image. These results suggested that the possibility of the crystal particle being
medical hydroxyapatite was high, and toothpaste containing medical hydroxyapatite
contributed to recrystalization of the decalcified enamel surface.

1920 A New Enamel Restoring Agent for Use after PMTC
M. NISHIO1, H. KAWMATA1, K. FUJITA1, T. ISHIZAKI1, R. HAYMAN1, and T. IKEMI2
1 Sangi Co., Ltd, Tokyo, Japan, 2 Nihon University at Matsudo, Japan

Objective:

 Professional mechanical tooth cleaning (PMTC), which uses abrasives
to remove biofilms and other surface deposits, also causes microscopic damage to
tooth enamel. This can lead to early return of plaque, and in fact increase the risk
of caries and periodontal disease. We developed and tested an agent designed to
restore tooth enamel to its original state after PMTC treatment.

Method:
Extracted human anterior teeth without previous restorative treatment, caries or
white-spot lesions were polished with a commonly used PMTC abrasive agent. The
teeth were then polished with a hydroxyapatite-based agent designed to restore the
enamel surface (PRTC Super Fine, SANGI). The enamel surface was observed
before PMTC, after PMTC, and after post-PMTC polishing with the enamel
restoring agent, using a scanning probe microscope (SPM) (SPI4000, Seiko
Instruments) and a scanning electron microscope (SEM) (S-4500, HITACHI). SPM
observation allowed both qualitative (three dimensional) and quantitative
(computed) evaluation of the enamel surface at each stage of processing. Result:
SPM observation showed some coarseness in the enamel surface prior to PMTC
treatment, believed to result from toothbrushing and other normal abrasion. The
enamel surface after PMTC treatment was much coarser, both qualitatively and
quantitatively, indicating that further damage to the surface enamel had occurred.
After post-PMTC polishing with the enamel restoring agent, the enamel surface
closely resembled that prior to PMTC treatment, suggesting that restoration of the
enamel had occurred. SEM observation confirmed that the enamel surface was
rougher after PMTC treatment than before, and that after post-PMTC treatment
with the new enamel restoring agent, it closely resembled the original enamel
surface.

Conclusion:

The hydroxyapatite-based agent tested effectively restores
post-PMTC tooth enamel to its near-original state. We called this new agent PRTC
(‘Professional Re-enamel Tooth Cleaning') Super Fine, to distinguish it from
traditional PMTC polishing agents.