A-PRF 논문

Optimized Platelet Rich Fibrin with the Low Speed Concept: Growth Factor Release, Biocompatibility and Cellular Response

 

Masako Fujioka-Kobayashi^1,2,3#, Richard J. Miron^1#*, Maria Hernandez¹, Umadevi Kandalam⁴, Yufeng Zhang⁵, Joseph Choukroun⁶

 

 

¹Department of Periodontology, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA; ²Department of Cranio-Maxillofacial Surgery, University of Bern, Bern, Switzerland; ³Department of Oral Surgery, Clinical Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; ¹Department of Pediatric Dentistry, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA ⁶Pain Clinic, Nice, France

 

#authors contributed equally to this work

 

* Corresponding author.

Richard Miron

Department of Periodontology

College of Dental Medicine

Nova Southeastern University

Fort Lauderdale, Florida, USA

rmiron@nova.edu

Abstract

Background: Over the past decade, the use of leukocyte platelet rich fibrin (L-PRF) has gained tremendous momentum in regenerative dentistry as a low-cost fibrin matrix utilized for tissue regeneration. In this study, we characterize how centrifugation speed (G-force) along with centrifugation time influence growth factor release from fibrin clots, as well as the cellular activity of gingival fibroblasts exposed to each PRF matrix.

 

Methods: Standard L-PRF served as a control (2700rpm-12 minutes). Two test groups utilizing low-speed (1300rpm-14 min termed advanced-PRF, A-PRF) and low-speed+time (1300rpm-8 min; A-PRF+) were investigated. Each PRF matrix was tested for growth factor release up to 10 days (8 patient samples) as well as biocompatibility and cellular activity.

 

Results: The low speed concept (A-PRF, A-PRF+) demonstrated a significant increase in growth factor release of PDGF, TGF- β1, EGF and IGF with A-PRF+ being highest of all groups. While all PRF formulations were extremely biocompatible due to their autogenous sources, both A-PRF and A-PRF+ demonstrated significantly higher levels of human fibroblast migration and proliferation when compared to L-PRF. Furthermore, gingival fibroblasts cultured with A-PRF+ demonstrated significantly higher mRNA levels of PDGF, TGF-β1 and collagen1 at either 3 or 7 days.

 

Conclusions: The findings from the present study demonstrate that modifications to centrifugation speed and time with the low-speed concept was shown to favor an increase in growth factor release from PRF clots which in turn may directly influence tissue regeneration by increasing fibroblast migration, proliferation and collagen synthesis. Future animal and clinical studies are now necessary.

 

 

 

Keywords: platelet rich fibrin, PRF, platelet concentrates, growth factor release, tissue regeneration, autogenous blood concentrates