염증 및 분화 관련 세포 반응에 대한 치과용 Mineral Trioxide Aggregate의 영향

염증 및 분화 관련 세포 반응에 대한 치과용 Mineral Trioxide Aggregate의 영향

Effects of Dental Mineral Trioxide Aggregate on Cellular Reactions
Involved in Inflammation and Differentiation
김종길·손경미·박희철·주정정·권지현·양형철*

Jong-Gil Kim, Kyung Mi Son, Hee Chul Park, Tingting Zhu, Ji Hyun Kwon, and Hyeong-Cheol Yang*
서울대학교 치의학대학원 치과생체재료과학교실 및 치학연구소 110-149, 서울특별시 종로구 연건동 28
Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry,
Seoul National University 28 Yongon-dong, Chonro-gu, Seoul 110-749, Korea
(Received January 21, 2013/Acccepted January 30, 2013)

Mineral trioxide aggregate (MTA) is receiving more attentions in endodontic dental clinics due to its high sealing ability
and biocompatibility. Although previous studies have reported the effects of MTA on dental pulp tissue, the mechanisms
underlying on inflammation and differentiation at cellular levels are not yet fully understood. In this study, we
investigated various molecular aspects of cellular reactions involved in inflammation and differentiation to commercially
available MTAs including ProRoot MTA, Ortho MTA and MTA-Angelus. mRNA of COX-2 gene which is involved in
inflammatory reaction was not increased by all tested MTAs, and ｅxpression of COX-2 by lipopolysaccharide was not
severely altered by MTAs, demonstrating that COX-2 is not involved in inflammatory reactions to MTA. The extracts
of all MTAs obtained for early 24 hrs increased ALP activities of human dental pulp (HDP) cells, while the extracts of
ProRoot MTA and MTA-Angelus obtained for 1-3 and 3-6 day decreased ALP activity, indicating the presence of inhibitory
components in the two MTAs. ProRoot MTA enhanced mRNA levels of DMP-1, but not DSPP. However, the other
MTAs did not affect mRNA ｅxpression of DSPP, DMP-1, OCN and OPN, which is not consistent with ALP activities.
Thus, further studies are needed to conclude the effects of MTA on differentiation of dental pulp cells.
Key words: mineral trioxide aggregate, cytotoxicity, cyclooxygenase-2, inflammation, differentiation